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paav gfaab nature metabolism article  (Addgene inc)


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    Addgene inc paav gfaab nature metabolism article
    Paav Gfaab Nature Metabolism Article, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paav+gfaab+nature+metabolism+article/CD63+GYEVI-pEGFP+C2+(Plasmid+%23101038)/pm41188644-335-16-26
    Average 94 stars, based on 73 article reviews
    paav gfaab nature metabolism article - by Bioz Stars, 2026-09
    94/100 stars

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    Sequencing:

    Article Title: Mitochondrial complex III-derived ROS amplify immunometabolic changes in astrocytes and promote dementia pathology.
    Article Snippet: BamHI-AOX-NheI and BamHI-AOXmut-NheI DNA sequences without stop codons were synthesized and cloned into pUC-Amp by Genewiz. .. Coexpression of AOX and mtHyPer7 in astrocytes was achieved by first replacing the NEUROD1 sequence in pAAV-GfaAB Nature Metabolism Article https://doi.org/10.1038/s42255-025-01390-y C1D-NEUROD1-T2A-mCherry (donated by C.-L. Zhang, Addgene plasmid 178582; RRID: Addgene_178582) with AOX or AOXmut using standard BamHI and NheI digestion and ligation to generate pAAV-hGfaABC1D-AOX-T2A-mCherry-WPRE-hGHpA and pAAV-hGfaAB C1D-AOXmut-T2A-mCherry-WPRE-hGHpA. mtHyPer7 was cloned in place of the mCherry reporter in these vectors by removing the AgeI-mCherry-HindIII and replacing with PCR-amplified and digested Eco88I- mtHyPer7-HindIII using Eco88I-containing forward primer = 5′-CTA CCC GGG ATG TCC GTC CTG ACG CCG and HindIII-containing reverse primer = 5′-ACT AAG CTT TCA ATC GCA GAT GAA GCT. ..

    Plasmid Preparation:

    Article Title: Mitochondrial complex III-derived ROS amplify immunometabolic changes in astrocytes and promote dementia pathology.
    Article Snippet: BamHI-AOX-NheI and BamHI-AOXmut-NheI DNA sequences without stop codons were synthesized and cloned into pUC-Amp by Genewiz. .. Coexpression of AOX and mtHyPer7 in astrocytes was achieved by first replacing the NEUROD1 sequence in pAAV-GfaAB Nature Metabolism Article https://doi.org/10.1038/s42255-025-01390-y C1D-NEUROD1-T2A-mCherry (donated by C.-L. Zhang, Addgene plasmid 178582; RRID: Addgene_178582) with AOX or AOXmut using standard BamHI and NheI digestion and ligation to generate pAAV-hGfaABC1D-AOX-T2A-mCherry-WPRE-hGHpA and pAAV-hGfaAB C1D-AOXmut-T2A-mCherry-WPRE-hGHpA. mtHyPer7 was cloned in place of the mCherry reporter in these vectors by removing the AgeI-mCherry-HindIII and replacing with PCR-amplified and digested Eco88I- mtHyPer7-HindIII using Eco88I-containing forward primer = 5′-CTA CCC GGG ATG TCC GTC CTG ACG CCG and HindIII-containing reverse primer = 5′-ACT AAG CTT TCA ATC GCA GAT GAA GCT. ..

    Ligation:

    Article Title: Mitochondrial complex III-derived ROS amplify immunometabolic changes in astrocytes and promote dementia pathology.
    Article Snippet: BamHI-AOX-NheI and BamHI-AOXmut-NheI DNA sequences without stop codons were synthesized and cloned into pUC-Amp by Genewiz. .. Coexpression of AOX and mtHyPer7 in astrocytes was achieved by first replacing the NEUROD1 sequence in pAAV-GfaAB Nature Metabolism Article https://doi.org/10.1038/s42255-025-01390-y C1D-NEUROD1-T2A-mCherry (donated by C.-L. Zhang, Addgene plasmid 178582; RRID: Addgene_178582) with AOX or AOXmut using standard BamHI and NheI digestion and ligation to generate pAAV-hGfaABC1D-AOX-T2A-mCherry-WPRE-hGHpA and pAAV-hGfaAB C1D-AOXmut-T2A-mCherry-WPRE-hGHpA. mtHyPer7 was cloned in place of the mCherry reporter in these vectors by removing the AgeI-mCherry-HindIII and replacing with PCR-amplified and digested Eco88I- mtHyPer7-HindIII using Eco88I-containing forward primer = 5′-CTA CCC GGG ATG TCC GTC CTG ACG CCG and HindIII-containing reverse primer = 5′-ACT AAG CTT TCA ATC GCA GAT GAA GCT. ..

    Clone Assay:

    Article Title: Mitochondrial complex III-derived ROS amplify immunometabolic changes in astrocytes and promote dementia pathology.
    Article Snippet: BamHI-AOX-NheI and BamHI-AOXmut-NheI DNA sequences without stop codons were synthesized and cloned into pUC-Amp by Genewiz. .. Coexpression of AOX and mtHyPer7 in astrocytes was achieved by first replacing the NEUROD1 sequence in pAAV-GfaAB Nature Metabolism Article https://doi.org/10.1038/s42255-025-01390-y C1D-NEUROD1-T2A-mCherry (donated by C.-L. Zhang, Addgene plasmid 178582; RRID: Addgene_178582) with AOX or AOXmut using standard BamHI and NheI digestion and ligation to generate pAAV-hGfaABC1D-AOX-T2A-mCherry-WPRE-hGHpA and pAAV-hGfaAB C1D-AOXmut-T2A-mCherry-WPRE-hGHpA. mtHyPer7 was cloned in place of the mCherry reporter in these vectors by removing the AgeI-mCherry-HindIII and replacing with PCR-amplified and digested Eco88I- mtHyPer7-HindIII using Eco88I-containing forward primer = 5′-CTA CCC GGG ATG TCC GTC CTG ACG CCG and HindIII-containing reverse primer = 5′-ACT AAG CTT TCA ATC GCA GAT GAA GCT. ..

    Polymerase Chain Reaction:

    Article Title: Mitochondrial complex III-derived ROS amplify immunometabolic changes in astrocytes and promote dementia pathology.
    Article Snippet: BamHI-AOX-NheI and BamHI-AOXmut-NheI DNA sequences without stop codons were synthesized and cloned into pUC-Amp by Genewiz. .. Coexpression of AOX and mtHyPer7 in astrocytes was achieved by first replacing the NEUROD1 sequence in pAAV-GfaAB Nature Metabolism Article https://doi.org/10.1038/s42255-025-01390-y C1D-NEUROD1-T2A-mCherry (donated by C.-L. Zhang, Addgene plasmid 178582; RRID: Addgene_178582) with AOX or AOXmut using standard BamHI and NheI digestion and ligation to generate pAAV-hGfaABC1D-AOX-T2A-mCherry-WPRE-hGHpA and pAAV-hGfaAB C1D-AOXmut-T2A-mCherry-WPRE-hGHpA. mtHyPer7 was cloned in place of the mCherry reporter in these vectors by removing the AgeI-mCherry-HindIII and replacing with PCR-amplified and digested Eco88I- mtHyPer7-HindIII using Eco88I-containing forward primer = 5′-CTA CCC GGG ATG TCC GTC CTG ACG CCG and HindIII-containing reverse primer = 5′-ACT AAG CTT TCA ATC GCA GAT GAA GCT. ..

    Countercurrent Chromatography:

    Article Title: Mitochondrial complex III-derived ROS amplify immunometabolic changes in astrocytes and promote dementia pathology.
    Article Snippet: BamHI-AOX-NheI and BamHI-AOXmut-NheI DNA sequences without stop codons were synthesized and cloned into pUC-Amp by Genewiz. .. Coexpression of AOX and mtHyPer7 in astrocytes was achieved by first replacing the NEUROD1 sequence in pAAV-GfaAB Nature Metabolism Article https://doi.org/10.1038/s42255-025-01390-y C1D-NEUROD1-T2A-mCherry (donated by C.-L. Zhang, Addgene plasmid 178582; RRID: Addgene_178582) with AOX or AOXmut using standard BamHI and NheI digestion and ligation to generate pAAV-hGfaABC1D-AOX-T2A-mCherry-WPRE-hGHpA and pAAV-hGfaAB C1D-AOXmut-T2A-mCherry-WPRE-hGHpA. mtHyPer7 was cloned in place of the mCherry reporter in these vectors by removing the AgeI-mCherry-HindIII and replacing with PCR-amplified and digested Eco88I- mtHyPer7-HindIII using Eco88I-containing forward primer = 5′-CTA CCC GGG ATG TCC GTC CTG ACG CCG and HindIII-containing reverse primer = 5′-ACT AAG CTT TCA ATC GCA GAT GAA GCT. ..



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    paav gfaab nature metabolism article  (Addgene inc)
    94
    Addgene inc paav gfaab nature metabolism article
    Paav Gfaab Nature Metabolism Article, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paav+gfaab+nature+metabolism+article/CD63+GYEVI-pEGFP+C2+(Plasmid+%23101038)/pm41188644-335-16-26
    Average 94 stars, based on 1 article reviews
    paav gfaab nature metabolism article - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

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